Hieff Unicon™ Universal Blue qPCR Master Mix (Dye Based) is a pre-solution for 2×real-time quantitative PCR amplification characterized by high sensitivity and specificity, is blue in color, and has the effect of sample addition
This product is a 2× Mix pre-mixed reagent that enables up to four fluorescent quantitative PCR reactions in a single reaction well. This product contains the genetically modified antibody method to hot-start Taq enzyme, greatly improving the amplification sensitivity and specificity. At the same time, this product has deeply optimized the multi-reaction buffer, which can improve the amplification efficiency of the reaction and promote the effective amplification of low-concentration templates. This product can be used for genotyping and multiplex quantitative analysis.
|Hot Start||Built-in hot start|
|Detection method||Primer-probe detection|
|Polymerase||Taq DNA polymerase|
|Type of sample||DNA|
|Application equipment||Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™, StepOne Plus™, 7500, 7500 Fast, ViiA™7, QuantStudio™ 3 and 5, QuantStudio™ 6,7,12k Flex; Bio-Rad: CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4; Eppendorf: Mastercycler ep realplex, realplex 2 s; Qiagen: Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000; Roche Applied Science: LightCycler 480, LightCycler 2.0; Lightcycler 96; Stratagene: MX3000P™, MX3005P™, MX4000P™; Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR.|
|11211||Hieff Unicon™Universal Multiplex qPCR Master Mix (Probe Based)||1 mL||5×1 mL||5 mL||20 mL||100×1 mL||100 mL|
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 2 year.
Figure 1 High stability: 37℃ thermal acceleration stability
The red line: 37℃ thermal acceleration 0 day; The yellow line: 37℃ thermal acceleration 7 days; The blue line: 37℃ thermal acceleration 14 days
Figure 2 High stability: freeze-thaw stability
The red line: freeze thaw o time; The yellow line: freeze thaw 50 times; The blue line: NTC
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